Excellent article: "Anatomy of an Ethernet Frame"

Trey Layton (aka EthernetStorageGuy) wrote excellent article about MTU sizes and Jumbo Frame settings. The article is here. In the article you will learn what MTU size parameters you have to configure in the path among server, network gear and storage. It is crucial to understand difference between payload (usually 1500 or 9000) and different frame sizes (usually 1522 or 9018 or 9022 or  9216) on networking equipment.

Here is summation of  Trey's deep Ethernet frame anatomy in to the simple best practice. "If you want to implement Jumbo Frames use pure datacenter networking equipment and setup MTU size to the device maximum which is usually 9216."

How to – use vmkping to verify Jumbo Frames

Here is nice blog post about Jumbo Frame configuration on vSphere and how to test it works as expected. This is BTW excellent test for Operational Verification (aka Test Plan).